Biotechnology - MCQ Practice Questions

In transgenic plant development, what is the primary function of the Ti plasmid from Agrobacterium tumefaciens?

Which crop was the first genetically modified organism (GMO) approved for commercial cultivation in India?

What does 'Bt' in Bt Cotton refer to?

Which Indian government body is primarily responsible for approving the release of genetically modified crops?

In the production of recombinant human insulin using genetically engineered bacteria, which organism is primarily used?

Which of the following enzymes is primarily used to cut DNA at specific recognition sequences during genetic engineering?

Which enzyme is responsible for synthesizing new DNA strands during PCR?

In PCR, the denaturation step typically occurs at:

Restriction enzymes recognize and cut DNA at:

The role of DNA ligase in genetic engineering is to:

The polymerase chain reaction was invented by:

The extension step in PCR occurs at approximately:

In PCR, which enzyme synthesizes the complementary DNA strand during the extension phase?

What is the typical number of PCR cycles required to achieve exponential amplification of target DNA?

Which of the following is NOT a component of the PCR reaction mixture?

In DNA sequencing by Sanger method, what role do ddNTPs play?

During PCR amplification, which enzyme synthesizes new DNA strands by reading the template DNA in the 3' to 5' direction?

What is the typical temperature range for the annealing step in a standard PCR cycle?

In real-time PCR (qPCR), which fluorescent dye binds directly to double-stranded DNA and is most commonly used in 2024-25 diagnostics?

What is the primary principle behind whole exome sequencing (WES) in identifying disease-causing mutations?