Biotechnology - MCQ Practice Questions

Which epigenetic modification is commonly exploited in epigenome editing to achieve stable gene silencing without altering DNA sequence?

What is the primary mechanism of action of TALENs (Transcription Activator-Like Effector Nucleases) in gene editing?

In next-generation sequencing (NGS), what is the primary advantage of whole-genome sequencing over targeted gene sequencing?

Which of the following represents a limitation of homologous recombination-based gene targeting?

In cloning, which vector is most suitable for inserting large DNA fragments (>15 kb)?

The principle of AFLP (Amplified Fragment Length Polymorphism) combines:

In gene editing, CRISPR-Cas9 uses which type of guide molecule?

In forensic DNA analysis, STR (Short Tandem Repeat) profiling is preferred over SNPs because:

Which technique combines PCR with capillary electrophoresis for high-resolution DNA fragment analysis?

In the context of 2024-25 genomic studies, what is the primary advantage of long-read sequencing technologies (PacBio, Oxford Nanopore) over short-read NGS?

What is the significance of the primer Tm (melting temperature) in multiplex PCR?

CRISPR-Cas12 (Cpf1) differs from Cas9 in that it:

In environmental metagenomic studies using 16S/18S rRNA amplicon sequencing, what is the primary limitation?

In somatic gene therapy using ex vivo approach, which cell type is most commonly targeted for genetic modification?

Which DNA technology is most suitable for detecting chromosomal abnormalities like deletions and duplications at the genomic level?

When designing a TaqMan probe for qPCR, what is the relationship between probe length and specificity in target detection?

In forensic DNA analysis using STR markers, what advantage do newer SNP-based methods provide over traditional STR typing for 2024-25 investigations?

Which PCR-based technique is specifically used to amplify and sequence unknown DNA flanking regions of known sequences?

In GWAS (Genome-Wide Association Studies) using DNA microarrays, what statistical principle is applied to identify significant SNP-trait associations?

What is the primary principle of High-Resolution Melting (HRM) analysis in qPCR?