What is the PAM (Protospacer Adjacent Motif) sequence requirement in Streptococcus pyogenes CRISPR-Cas9?
Q.42Medium
In transcription factor-based gene regulation, which domain is responsible for DNA binding?
Q.43Medium
Which of the following is an example of a selectable marker gene used in genetic engineering?
Q.44Medium
In gene therapy, what is the primary advantage of using adeno-associated virus (AAV) as a vector?
Q.45Medium
What is the significance of the TATA box in eukaryotic gene expression?
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Q.46Medium
Which chromosomal engineering technique is used to determine the order and orientation of genes on a chromosome?
Q.47Medium
In the context of RNA interference (RNAi), what is the role of the RISC complex?
Q.48Medium
A biotechnology company in India has developed a transgenic crop that produces its own Bt toxin for pest resistance. Which of the following best explains the mechanism by which this toxin controls insect pests?
Q.49Medium
In CRISPR-Cas9 gene editing technology, what is the specific role of the single guide RNA (sgRNA)?
Q.50Medium
During the development of a genetically modified organism (GMO), scientists use selectable marker genes. If an antibiotic resistance gene is used as a marker, which scenario would indicate successful transformation?
Q.51Medium
The optimal annealing temperature in PCR is typically set based on:
Q.52Medium
Which of the following is a limitation of conventional PCR?
Q.53Medium
What is the primary advantage of real-time PCR (qPCR) over conventional PCR?
Q.54Medium
Which type of restriction enzyme produces 'sticky ends'?
Q.55Medium
In RAPD-PCR, the primers are:
Q.56Medium
Which technique is most suitable for detecting SNPs (Single Nucleotide Polymorphisms)?
Q.57Medium
In DNA fingerprinting, which polymorphic markers are most commonly used in India?
Q.58Medium
Which of the following is a common application of PCR in diagnostic medicine?
Q.59Medium
What is the significance of dNTPs in PCR?
Q.60Medium
Real-time PCR differs from conventional PCR in that it:
