Biotechnology - MCQ Practice Questions

Which annealing temperature is most appropriate for primers with a GC content of 50%?

In next-generation sequencing (NGS), what is the primary advantage of massively parallel sequencing over conventional Sanger sequencing?

What is the purpose of using a 'hot start' Taq polymerase in PCR?

In DNA barcoding for species identification, which gene region is most commonly used?

Which of the following best describes the principle of digital PCR (dPCR)?

In gene therapy, AAV (Adeno-associated virus) vectors are preferred because they:

What is the principle behind site-directed mutagenesis using PCR?

Which PCR variant is specifically designed for amplifying and detecting microRNA (miRNA) targets in clinical samples?

In RAPD-PCR (Random Amplified Polymorphic DNA), what is the primary application in agricultural biotechnology?

During DNA amplification using PCR, if the initial template has 2 copies, approximately how many copies will be present after 30 cycles (assuming 100% efficiency)?

What is the main disadvantage of using standard Taq polymerase compared to high-fidelity polymerases like Pfu in PCR?

In multiplex PCR, what is the critical challenge that must be addressed when designing multiple primer sets?

Which DNA technology technique combines random DNA fragmentation with sequence-independent single-primer amplification (SISPA)?

In droplet digital PCR (ddPCR), how does partitioning of the sample improve quantification accuracy compared to conventional qPCR?

For amplifying extremely long DNA fragments (>10 kb), which specialized PCR technique is most appropriate?

In CRISPR-Cas9 gene editing, what is the function of the PAM (Protospacer Adjacent Motif) sequence?

In next-generation sequencing (NGS) library preparation, what is the primary purpose of adapter ligation in DNA technology workflows?

In the context of DNA barcode libraries for species identification in 2024-25, which universal marker is most commonly used for plants?

Real-time PCR (qPCR) differs from conventional PCR in that it:

Which of the following is a common application of multiplex PCR?