Biotechnology - MCQ Practice Questions

In reverse transcription PCR (RT-PCR), which enzyme is critical for the first step?

What is the primary advantage of digital PCR (dPCR) over conventional qPCR?

AFLP (Amplified Fragment Length Polymorphism) technology combines which two DNA techniques?

In forensic applications, why are microsatellites (STRs) preferred over SNPs for individual identification?

What is the primary limitation of PCR when amplifying GC-rich sequences?

In the diagnosis of genetic disorders, why is allele-specific PCR (AS-PCR) preferred for detecting known mutations?

What is the primary advantage of using nested PCR in pathogen detection?

In whole genome amplification (WGA) using multiple displacement amplification (MDA), which enzyme is used?

Which of the following statements about primer design for PCR is INCORRECT?

In real-time PCR (qPCR), which fluorescent dye binds to double-stranded DNA and is commonly used for target quantification?

Which PCR variant would be most suitable for amplifying DNA from degraded or partially degraded samples such as archaeological or forensic specimens?

In AFLP (Amplified Fragment Length Polymorphism) analysis, what are the two restriction enzymes typically used?

What is the primary disadvantage of using conventional PCR for pathogen detection in clinical diagnostics compared to real-time PCR?

In the context of human disease diagnosis, what does the Ct value (Cycle threshold) in qPCR represent?

Which DNA fingerprinting technique using PCR amplification of repetitive sequences is widely used in paternity testing and criminal investigations in India?

In homozygous versus heterozygous allele detection using allele-specific PCR, which outcome indicates a heterozygous genotype?

In the context of environmental microbiology, what does PCR-DGGE (Denaturing Gradient Gel Electrophoresis) primarily assess?

In next-generation sequencing library preparation, why is PCR amplification of adaptor-ligated DNA fragments critical?

During PCR amplification, the annealing temperature is set based on the Tm (melting temperature) of primers. If primers with Tm of 58°C and 62°C are used in the same reaction, what would be the optimal annealing temperature to ensure efficient amplification of both targets?

In a multiplex PCR assay designed to simultaneously amplify 6 different STR (short tandem repeat) loci for forensic DNA profiling, what is the critical consideration that must be addressed?