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Biotechnology - MCQ Practice Questions

Practice free Biotechnology multiple-choice questions with detailed answers and explanations. Perfect for competitive exam preparation.

260 questions | 100% Free

Q.1Medium

Which hormone is most critical for cell growth and proliferation in serum-containing culture media?

Q.2Medium

What is the significance of calculating the cell doubling time in cell culture?

Q.3Medium

Which of the following is NOT a method for cryopreservation of cell cultures?

Q.4Medium

What is the Hayflick limit?

Q.5Medium

Which technique is used to determine cell viability immediately after thawing from cryopreservation?

Q.6Medium

What is the primary advantage of using serum-free media in cell culture?

Q.7Medium

Which of the following best describes contact inhibition in normal cell cultures?

Q.8Medium

An animal cell culture shows signs of contamination with bacteria. Which characteristic would NOT be observed?

Q.9Medium

What is the optimal CO₂ concentration typically maintained in mammalian cell culture incubators?

Q.10Medium

Which of the following applications of cell culture is most relevant for drug testing in India's pharmaceutical industry (2024-2025)?

Q.11Medium

Which parameter is most critical for maintaining pH stability in a large-scale bioreactor?

Q.12Medium

What is the significance of cryopreservation in cell culture banking?

Q.13Medium

In the context of Indian pharmaceutical biotech (2024-25), which cell line is most commonly used for recombinant protein production?

Q.14Medium

Which of the following best describes the G0 phase in the cell cycle?

Q.15Medium

What is the primary advantage of using suspension culture systems over adherent culture for large-scale production?

Q.16Medium

During scale-up of cell culture from 2L to 200L bioreactor, which parameter becomes increasingly challenging to maintain?

Q.17Medium

What is the primary function of osmolarity/osmotic pressure control in cell culture media?

Q.18Medium

In viral vaccine production using cell culture, which cell line is commonly used for influenza vaccine manufacturing?

Q.19Medium

What is the role of trypsin in cell culture preparation and maintenance?

Q.20Medium

Which of the following statements about lactate production in mammalian cell culture is correct?