Biotechnology - MCQ Practice Questions

Which enzyme is responsible for synthesizing new DNA strands during PCR?

In PCR, the denaturation step typically occurs at:

Restriction enzymes recognize and cut DNA at:

The role of DNA ligase in genetic engineering is to:

The polymerase chain reaction was invented by:

The extension step in PCR occurs at approximately:

In PCR, which enzyme synthesizes the complementary DNA strand during the extension phase?

What is the typical number of PCR cycles required to achieve exponential amplification of target DNA?

Which of the following is NOT a component of the PCR reaction mixture?

In DNA sequencing by Sanger method, what role do ddNTPs play?

During PCR amplification, which enzyme synthesizes new DNA strands by reading the template DNA in the 3' to 5' direction?

What is the typical temperature range for the annealing step in a standard PCR cycle?

In real-time PCR (qPCR), which fluorescent dye binds directly to double-stranded DNA and is most commonly used in 2024-25 diagnostics?

What is the primary principle behind whole exome sequencing (WES) in identifying disease-causing mutations?

What is the optimal annealing temperature range for most PCR reactions?

Which thermostable DNA polymerase is most commonly used in standard PCR?

In a typical PCR cycle, DNA denaturation occurs at approximately which temperature?

What is the primary function of dNTPs in PCR?

Which enzyme is primarily responsible for extending DNA strands during the elongation phase of PCR?

In a standard PCR cycle, at what temperature is the DNA double helix typically denatured?