Biotechnology - MCQ Practice Questions

Which enzyme is responsible for synthesizing new DNA strands during PCR?

The optimal annealing temperature in PCR is typically set based on:

In PCR, the denaturation step typically occurs at:

Which of the following is a limitation of conventional PCR?

What is the primary advantage of real-time PCR (qPCR) over conventional PCR?

Restriction enzymes recognize and cut DNA at:

Which type of restriction enzyme produces 'sticky ends'?

The role of DNA ligase in genetic engineering is to:

In RAPD-PCR, the primers are:

Which technique is most suitable for detecting SNPs (Single Nucleotide Polymorphisms)?

The polymerase chain reaction was invented by:

In DNA fingerprinting, which polymorphic markers are most commonly used in India?

The extension step in PCR occurs at approximately:

Which of the following is a common application of PCR in diagnostic medicine?

What is the significance of dNTPs in PCR?

In cloning, which vector is most suitable for inserting large DNA fragments (>15 kb)?

The principle of AFLP (Amplified Fragment Length Polymorphism) combines:

In gene editing, CRISPR-Cas9 uses which type of guide molecule?

In PCR, which enzyme synthesizes the complementary DNA strand during the extension phase?

What is the typical number of PCR cycles required to achieve exponential amplification of target DNA?