A researcher used CRISPR-Cas9 to edit a disease-causing mutation in human embryo cells. What ethical concern is most relevant?
Q.2Hard
A biotechnology company wants to develop a drought-resistant wheat variety using genetic engineering. Which genes would be most suitable to introduce?
Q.3Hard
In the development of Bt Brinjal in India, which Bacillus thuringiensis gene was primarily used and for which pest resistance?
Q.4Hard
What is the primary limitation of using bacterial plasmids as vectors for introducing large genes (>50 kb) into eukaryotic cells?
Q.5Hard
A genetically modified organism (GMO) shows unexpected phenotypic changes not related to the introduced transgene. What biological phenomenon best explains this?
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Q.6Hard
A researcher wants to produce recombinant human insulin in bacteria. Which of the following steps would be MOST critical for ensuring successful protein expression?
Q.7Hard
Which modern technique allows for the creation of gene edits that can be reversed or made inducible, offering greater control over gene expression?
Q.8Hard
Consider a scenario where a genetic engineer develops a drought-resistant crop by introducing an aquaporin gene. Which of the following would be the most appropriate long-term assessment of this GM crop's environmental impact?
Q.9Hard
In gene therapy for genetic disorders, what is the primary advantage of using ex vivo gene therapy compared to in vivo therapy?
Q.10Hard
Which of the following represents the most significant biosafety concern regarding horizontal gene transfer from GM crops to wild relatives?
Q.11Hard
A biotechnology company successfully edits a disease-causing gene in human embryonic stem cells using CRISPR-Cas9. Before proceeding with clinical trials, what is the primary regulatory and ethical challenge they would face in India?
Q.12Hard
What is the primary mechanism by which RNA interference (RNAi) achieves gene silencing?
Q.13Hard
In next-generation sequencing-based CRISPR screening, what does 'pooled library screening' allow researchers to identify?
Q.14Hard
Which of the following represents the most significant challenge in applying base editing technology for therapeutic applications?
Q.15Hard
What is the significance of using 'prime editing' technology over conventional CRISPR-Cas9 in genetic engineering applications?
Q.16Hard
In zinc finger nuclease (ZFN) technology, how many zinc finger domains are typically required to recognize and bind to a specific DNA sequence?
Q.17Hard
Which of the following would be the BEST strategy to minimize off-target effects in CRISPR-Cas9 editing of human embryonic stem cells?
Q.18Hard
In the context of gene therapy vectors, which characteristic makes adeno-associated viruses (AAVs) preferable to lentiviruses for CNS diseases?
Q.19Hard
Which technique combines microfluidics with next-generation sequencing to analyze single-cell genetic modifications in heterogeneous populations?
Q.20Hard
In base editing technology, what is the critical difference between cytidine and adenosine deaminases in terms of target DNA modification?
