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Biotechnology - MCQ Practice Questions

Practice free Biotechnology multiple-choice questions with detailed answers and explanations. Perfect for competitive exam preparation.

260 questions | 100% Free

Q.1Easy

Which enzyme is commonly used to cut DNA at specific recognition sequences during genetic engineering?

Q.2Easy

What is the primary purpose of using DNA ligase in genetic engineering?

Q.3Easy

Which of the following is an example of a vector used in genetic engineering?

Q.4Easy

What is the main advantage of using Bt cotton in India?

Q.5Easy

Which Indian-approved genetically modified crop is widely cultivated for herbicide resistance?

Q.6Medium

The process of introducing foreign DNA into a plant cell using Agrobacterium tumefaciens is known as:

Q.7Medium

In CRISPR-Cas9 gene editing, what does the 'guide RNA' do?

Q.8Medium

Which of the following statements about Golden Rice is correct?

Q.9Medium

What is the role of a 'selectable marker' gene in genetic engineering?

Q.10Medium

In the context of genetic engineering, what does 'homologous recombination' mean?

Q.11Medium

Which technique would be most suitable for introducing multiple genes simultaneously into a plant cell?

Q.12Medium

What is the major concern regarding the long-term cultivation of Bt cotton in India?

Q.13Medium

Consider a genetic engineer wants to produce insulin using genetically modified bacteria. Which of the following is the correct sequence of steps?

Q.14Medium

Which of the following regulatory bodies in India is responsible for the approval of GM crops?

Q.15Hard

A researcher used CRISPR-Cas9 to edit a disease-causing mutation in human embryo cells. What ethical concern is most relevant?

Q.16Hard

A biotechnology company wants to develop a drought-resistant wheat variety using genetic engineering. Which genes would be most suitable to introduce?

Q.17Hard

In the development of Bt Brinjal in India, which Bacillus thuringiensis gene was primarily used and for which pest resistance?

Q.18Hard

What is the primary limitation of using bacterial plasmids as vectors for introducing large genes (>50 kb) into eukaryotic cells?

Q.19Hard

A genetically modified organism (GMO) shows unexpected phenotypic changes not related to the introduced transgene. What biological phenomenon best explains this?

Q.20Easy

Which enzyme is commonly used as a molecular scissors in CRISPR-Cas9 gene editing technology?