In cloning, which vector is most suitable for inserting large DNA fragments (>15 kb)?
Q.2Hard
The principle of AFLP (Amplified Fragment Length Polymorphism) combines:
Q.3Hard
In gene editing, CRISPR-Cas9 uses which type of guide molecule?
Q.4Hard
In forensic DNA analysis, STR (Short Tandem Repeat) profiling is preferred over SNPs because:
Q.5Hard
Which technique combines PCR with capillary electrophoresis for high-resolution DNA fragment analysis?
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Q.6Hard
In the context of 2024-25 genomic studies, what is the primary advantage of long-read sequencing technologies (PacBio, Oxford Nanopore) over short-read NGS?
Q.7Hard
What is the significance of the primer Tm (melting temperature) in multiplex PCR?
Q.8Hard
CRISPR-Cas12 (Cpf1) differs from Cas9 in that it:
Q.9Hard
In environmental metagenomic studies using 16S/18S rRNA amplicon sequencing, what is the primary limitation?
Q.10Hard
In somatic gene therapy using ex vivo approach, which cell type is most commonly targeted for genetic modification?
Q.11Hard
Which DNA technology is most suitable for detecting chromosomal abnormalities like deletions and duplications at the genomic level?
Q.12Hard
When designing a TaqMan probe for qPCR, what is the relationship between probe length and specificity in target detection?
Q.13Hard
In forensic DNA analysis using STR markers, what advantage do newer SNP-based methods provide over traditional STR typing for 2024-25 investigations?
Q.14Hard
Which PCR-based technique is specifically used to amplify and sequence unknown DNA flanking regions of known sequences?
Q.15Hard
In GWAS (Genome-Wide Association Studies) using DNA microarrays, what statistical principle is applied to identify significant SNP-trait associations?
Q.16Hard
What is the primary principle of High-Resolution Melting (HRM) analysis in qPCR?
Q.17Hard
In competitive PCR, what is the role of the internal control DNA?
Q.18Hard
Which PCR variant is most suitable for detecting point mutations in genomic DNA?
Q.19Hard
In the context of DNA barcoding for species identification, which gene region is commonly used for plants?
Q.20Hard
In SNP genotyping using TaqMan chemistry, what is the function of MGB (Minor Groove Binder)?
