What is the PAM (Protospacer Adjacent Motif) sequence requirement in Streptococcus pyogenes CRISPR-Cas9?
Q.42Medium
In transcription factor-based gene regulation, which domain is responsible for DNA binding?
Q.43Medium
Which of the following is an example of a selectable marker gene used in genetic engineering?
Q.44Medium
In gene therapy, what is the primary advantage of using adeno-associated virus (AAV) as a vector?
Q.45Medium
What is the significance of the TATA box in eukaryotic gene expression?
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Q.46Medium
Which chromosomal engineering technique is used to determine the order and orientation of genes on a chromosome?
Q.47Medium
In the context of RNA interference (RNAi), what is the role of the RISC complex?
Q.48Medium
A biotechnology company in India has developed a transgenic crop that produces its own Bt toxin for pest resistance. Which of the following best explains the mechanism by which this toxin controls insect pests?
Q.49Medium
In CRISPR-Cas9 gene editing technology, what is the specific role of the single guide RNA (sgRNA)?
Q.50Medium
During the development of a genetically modified organism (GMO), scientists use selectable marker genes. If an antibiotic resistance gene is used as a marker, which scenario would indicate successful transformation?