Biotechnology - MCQ Practice Questions

The optimal annealing temperature in PCR is typically set based on:

Which of the following is a limitation of conventional PCR?

What is the primary advantage of real-time PCR (qPCR) over conventional PCR?

Which type of restriction enzyme produces 'sticky ends'?

In RAPD-PCR, the primers are:

Which technique is most suitable for detecting SNPs (Single Nucleotide Polymorphisms)?

In DNA fingerprinting, which polymorphic markers are most commonly used in India?

Which of the following is a common application of PCR in diagnostic medicine?

What is the significance of dNTPs in PCR?

Real-time PCR differs from conventional PCR in that it:

Which annealing temperature is most appropriate for primers with a GC content of 50%?

In next-generation sequencing (NGS), what is the primary advantage of massively parallel sequencing over conventional Sanger sequencing?

What is the purpose of using a 'hot start' Taq polymerase in PCR?

In DNA barcoding for species identification, which gene region is most commonly used?

Which of the following best describes the principle of digital PCR (dPCR)?

In gene therapy, AAV (Adeno-associated virus) vectors are preferred because they:

What is the principle behind site-directed mutagenesis using PCR?

Which PCR variant is specifically designed for amplifying and detecting microRNA (miRNA) targets in clinical samples?

In RAPD-PCR (Random Amplified Polymorphic DNA), what is the primary application in agricultural biotechnology?

During DNA amplification using PCR, if the initial template has 2 copies, approximately how many copies will be present after 30 cycles (assuming 100% efficiency)?