Golden Rice, developed through genetic engineering, is primarily enriched with which nutrient?
Q.22Easy
What is the primary function of a restriction endonuclease in genetic engineering?
Q.23Easy
Which of the following is NOT a vector commonly used in genetic engineering?
Q.24Easy
In India, which regulatory body is responsible for approving genetically modified crops before their commercial release?
Q.25Medium
What is the primary advantage of using homologous recombination over random integration in genetic engineering?
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Q.26Medium
Bt Brinjal, developed in India, produces a toxin that is toxic to which major pest?
Q.27Medium
Which Indian state first approved the cultivation of Bt Cotton commercially?
Q.28Medium
In the process of DNA sequencing using Sanger's method, what does the term 'dideoxynucleotides' refer to?
Q.29Medium
What is the primary concern associated with the use of antibiotic resistance genes as selectable markers in genetic engineering?
Q.30Medium
Which of the following techniques would be most appropriate for introducing multiple genes simultaneously into a plant genome?
Q.31Medium
In CRISPR-Cas9 technology, what is the role of the single guide RNA (sgRNA)?
Q.32Medium
Which of the following best describes 'off-target effects' in CRISPR-Cas9 gene editing?
Q.33Hard
A researcher wants to produce recombinant human insulin in bacteria. Which of the following steps would be MOST critical for ensuring successful protein expression?
Q.34Hard
Which modern technique allows for the creation of gene edits that can be reversed or made inducible, offering greater control over gene expression?
Q.35Hard
Consider a scenario where a genetic engineer develops a drought-resistant crop by introducing an aquaporin gene. Which of the following would be the most appropriate long-term assessment of this GM crop's environmental impact?
Q.36Hard
In gene therapy for genetic disorders, what is the primary advantage of using ex vivo gene therapy compared to in vivo therapy?
Q.37Hard
Which of the following represents the most significant biosafety concern regarding horizontal gene transfer from GM crops to wild relatives?
Q.38Hard
A biotechnology company successfully edits a disease-causing gene in human embryonic stem cells using CRISPR-Cas9. Before proceeding with clinical trials, what is the primary regulatory and ethical challenge they would face in India?
Q.39Easy
Which enzyme is responsible for cutting DNA at specific recognition sequences during genetic engineering?
Q.40Easy
What is the primary function of DNA ligase in genetic engineering protocols?
