Biotechnology - MCQ Practice Questions

Which of the following is NOT a component of the PCR reaction mixture?

In DNA sequencing by Sanger method, what role do ddNTPs play?

Real-time PCR differs from conventional PCR in that it:

Which annealing temperature is most appropriate for primers with a GC content of 50%?

In next-generation sequencing (NGS), what is the primary advantage of massively parallel sequencing over conventional Sanger sequencing?

What is the purpose of using a 'hot start' Taq polymerase in PCR?

In DNA barcoding for species identification, which gene region is most commonly used?

Which of the following best describes the principle of digital PCR (dPCR)?

In gene therapy, AAV (Adeno-associated virus) vectors are preferred because they:

What is the principle behind site-directed mutagenesis using PCR?

In forensic DNA analysis, STR (Short Tandem Repeat) profiling is preferred over SNPs because:

Which technique combines PCR with capillary electrophoresis for high-resolution DNA fragment analysis?

In the context of 2024-25 genomic studies, what is the primary advantage of long-read sequencing technologies (PacBio, Oxford Nanopore) over short-read NGS?

What is the significance of the primer Tm (melting temperature) in multiplex PCR?

CRISPR-Cas12 (Cpf1) differs from Cas9 in that it:

In environmental metagenomic studies using 16S/18S rRNA amplicon sequencing, what is the primary limitation?

In somatic gene therapy using ex vivo approach, which cell type is most commonly targeted for genetic modification?

During PCR amplification, which enzyme synthesizes new DNA strands by reading the template DNA in the 3' to 5' direction?

What is the typical temperature range for the annealing step in a standard PCR cycle?

In real-time PCR (qPCR), which fluorescent dye binds directly to double-stranded DNA and is most commonly used in 2024-25 diagnostics?