Which of the following is a homologous recombination-based gene editing technique?
Q.82Medium
What is the PAM (Protospacer Adjacent Motif) sequence requirement in Streptococcus pyogenes CRISPR-Cas9?
Q.83Easy
In transgenic plant development, what is the primary function of the Ti plasmid from Agrobacterium tumefaciens?
Q.84Easy
Which crop was the first genetically modified organism (GMO) approved for commercial cultivation in India?
Q.85Easy
What does 'Bt' in Bt Cotton refer to?
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Q.86Medium
In transcription factor-based gene regulation, which domain is responsible for DNA binding?
Q.87Medium
Which of the following is an example of a selectable marker gene used in genetic engineering?
Q.88Medium
In gene therapy, what is the primary advantage of using adeno-associated virus (AAV) as a vector?
Q.89Medium
What is the significance of the TATA box in eukaryotic gene expression?
Q.90Medium
Which chromosomal engineering technique is used to determine the order and orientation of genes on a chromosome?
Q.91Medium
In the context of RNA interference (RNAi), what is the role of the RISC complex?
Q.92Easy
Which Indian government body is primarily responsible for approving the release of genetically modified crops?
Q.93Hard
What is the primary mechanism of action of TALENs (Transcription Activator-Like Effector Nucleases) in gene editing?
Q.94Hard
In next-generation sequencing (NGS), what is the primary advantage of whole-genome sequencing over targeted gene sequencing?
Q.95Hard
Which of the following represents a limitation of homologous recombination-based gene targeting?
Q.96Easy
In the production of recombinant human insulin using genetically engineered bacteria, which organism is primarily used?
Q.97Easy
Which of the following enzymes is primarily used to cut DNA at specific recognition sequences during genetic engineering?
Q.98Medium
A biotechnology company in India has developed a transgenic crop that produces its own Bt toxin for pest resistance. Which of the following best explains the mechanism by which this toxin controls insect pests?
Q.99Medium
In CRISPR-Cas9 gene editing technology, what is the specific role of the single guide RNA (sgRNA)?
Q.100Medium
During the development of a genetically modified organism (GMO), scientists use selectable marker genes. If an antibiotic resistance gene is used as a marker, which scenario would indicate successful transformation?
