Biotechnology - MCQ Practice Questions

In Bt cotton cultivation in India, the Cry1Ac toxin primarily targets which insect order?

Which technique is used to identify whether a gene sequence contains introns and exons in eukaryotic organisms?

According to India's National Biotechnology Development Strategy 2024, which sector receives the highest allocation for gene therapy research?

In homologous recombination-based gene editing, what is the primary advantage of using long homology arms (>1 kb) flanking the insert?

Which of the following genetically modified crops has been approved for commercial cultivation in India as per GEAC regulations (2024)?

In zinc finger nuclease (ZFN) technology, how many zinc finger domains are typically required to recognize and bind to a specific DNA sequence?

Which of the following would be the BEST strategy to minimize off-target effects in CRISPR-Cas9 editing of human embryonic stem cells?

In the context of gene therapy vectors, which characteristic makes adeno-associated viruses (AAVs) preferable to lentiviruses for CNS diseases?

Which regulatory body in India is responsible for approving genetically modified organisms under the Environment Protection Act, 1986?

In polymerase chain reaction (PCR), what is the primary function of the annealing temperature optimization?

Which technique combines microfluidics with next-generation sequencing to analyze single-cell genetic modifications in heterogeneous populations?

In insulin production using genetically engineered E. coli, why is the use of proinsulin preferred over direct insulin gene expression?

Which of the following represents an application of TALEN technology that has shown clinical promise in 2024?

In base editing technology, what is the critical difference between cytidine and adenosine deaminases in terms of target DNA modification?

According to India's DBT 2024 guidelines, what is the mandatory holding period for biosafety assessment of genetically modified crops before large-scale field trials?

Which epigenetic modification is commonly exploited in epigenome editing to achieve stable gene silencing without altering DNA sequence?

In synthetic biology, what is the primary advantage of using BioBricks standardized parts over conventional genetic engineering?

What is the role of DNA ligase in genetic engineering?

Which vector is most commonly used for large DNA inserts (up to 45 kb) in genetic engineering?

In CRISPR-Cas9 technology, what is the function of the guide RNA (gRNA)?